Optimization on the Stability of Linseed Oil-in-Water Nanoemulsions Generated by Ultrasonic Emulsification Using Response Surface Methodology (RSM)

Oil-in-water (O/W) nanoemulsions formed from bioactive compounds such as polyunsaturated fatty acids (PUFA), are commercially attractive because of the potential health benefits associated with their consumption. Emulsions with a mean droplet size as small as 72±5.14 nm and viscosity 3.84±0.26 mPa·s were achieved using a mixture of 10:100 (V/V) linseed oil and water in the presence of surfactants i.e. Tween 80 and Span 60 with the HLB of 11.7. The zeta potential of the generated nanoemulsion was approximately -18 mV at its original pH of 5.9. Results of this study indicated that desired zeta potential might be achieved by changing the pH of final emulsion.